t ni insect cells Search Results


d biotin  (Chem Impex International)
95
Chem Impex International d biotin
D Biotin, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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thiazolyl blue tetrazolium bromide  (Chem Impex International)
95
Chem Impex International thiazolyl blue tetrazolium bromide
Thiazolyl Blue Tetrazolium Bromide, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2 oxoglutarate  (Chem Impex International)
95
Chem Impex International 2 oxoglutarate
( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - <t>2-oxoglutarate,</t> EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).
2 Oxoglutarate, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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glycidyloxy propyltrimethoxysilane  (Chem Impex International)
95
Chem Impex International glycidyloxy propyltrimethoxysilane
( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - <t>2-oxoglutarate,</t> EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).
Glycidyloxy Propyltrimethoxysilane, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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folic acid  (Chem Impex International)
95
Chem Impex International folic acid
( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - <t>2-oxoglutarate,</t> EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).
Folic Acid, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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folic acid - by Bioz Stars, 2026-09
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l aspartic acid ß tert butylester  (Chem Impex International)
95
Chem Impex International l aspartic acid ß tert butylester
( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - <t>2-oxoglutarate,</t> EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).
L Aspartic Acid ß Tert Butylester, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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l aspartic acid ß tert butylester - by Bioz Stars, 2026-09
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succinic acid  (Chem Impex International)
95
Chem Impex International succinic acid
( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - <t>2-oxoglutarate,</t> EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).
Succinic Acid, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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succinic acid - by Bioz Stars, 2026-09
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chemicals l  (Chem Impex International)
95
Chem Impex International chemicals l
( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - <t>2-oxoglutarate,</t> EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).
Chemicals L, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chemicals l - by Bioz Stars, 2026-09
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macrospin columns  (Chem Impex International)
95
Chem Impex International macrospin columns
( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - <t>2-oxoglutarate,</t> EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).
Macrospin Columns, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+ni+insect+cells/Niacinamide/pm33305953__cb0c00900_si_001-26-0-19
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macrospin columns - by Bioz Stars, 2026-09
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t. ni bti-tn-5b-4 insect cell line  (AstraZeneca ltd)
90
AstraZeneca ltd t. ni bti-tn-5b-4 insect cell line
( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - <t>2-oxoglutarate,</t> EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).
T. Ni Bti Tn 5b 4 Insect Cell Line, supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/t+ni+insect+cells/t++ni+bti+tn+5b+4+insect+cell+line/pm15806353-52-1-9
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tnmefh insect medium  (Gemini Bio)
86
Gemini Bio tnmefh insect medium
( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - <t>2-oxoglutarate,</t> EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).
Tnmefh Insect Medium, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - 2-oxoglutarate, EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).

Journal: Journal of the American Chemical Society

Article Title: Biosynthesis of 14-membered cyclopeptide alkaloids via non-heme-iron- and 2-oxoglutarate-dependent oxidative decarboxylation

doi: 10.1021/jacs.5c23400

Figure Lengend Snippet: ( A ) EIC traces of 3 and decarboxylated cyFPIY ( 4 ) in methanolic extracts of transgenic N. benthamiana leaves expressing ZjuPrec-(FPIY) 3 -ZjuBURP with or without candidate decarboxylase ZjuDC for 7 days. ( B ) In vitro reconstitution of ZjuDC activity in the presence of Fe(II) and 2OG. ( C ) In vitro cofactor and substrate screening assays of ZjuDC. ( D ) ZjuDC reaction. ( E ) Kinetic characterization of ZjuDC with substrate 3 . Abbreviations: 2OG - 2-oxoglutarate, EIC – extracted ion chromatogram. ( F ) ZjuDC reaction with dimethylated substrate N,N -Me 2 -cyFPIY ( 6 ).

Article Snippet: Assays were 50 μL total volume with final concentrations of 50 mM HEPES pH 7.5, 200 μM FeSO 4 -7H 2 O, 500 μM 2-oxoglutarate (Chem-Impex Int’l Inc.), 400 μM L-ascorbic acid sodium salt (Acros Organics), 4% dimethyl sulfoxide, and 90 μM substrate 3 .

Techniques: Transgenic Assay, Expressing, In Vitro, Activity Assay